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hiseq rapid duo cbot sample loading kit  (New England Biolabs)


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    Structured Review

    New England Biolabs hiseq rapid duo cbot sample loading kit
    Hiseq Rapid Duo Cbot Sample Loading Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sample+loading+kit/pm34658683-62-15-23
    Average 86 stars, based on 1 article reviews
    hiseq rapid duo cbot sample loading kit - by Bioz Stars, 2026-11
    86/100 stars

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    Related Articles

    Incubation:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Convection:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Concentration Assay:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Chromatography:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Standard Deviation:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Agarose Gel Electrophoresis:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Migration:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Staining:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Fluorescence:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Gene Expression:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Immunoprecipitation:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Immunostaining:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Software:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    CCK-8 Assay:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Clone Assay:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Flow Cytometry:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    DC Protein Assay:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Stable Transfection:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Immunocytochemistry:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    FACS:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Methylation:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Protease Inhibitor:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Infection:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Sonication:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Mutagenesis:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    DNA Purification:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Luciferase:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Mass Spectrometry:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Reporter Assay:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Sequencing:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Purification:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Nucleic Acid Electrophoresis:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Plasmid Preparation:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    In Vitro:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Cell Culture:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Molecular Weight:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Quantitative RT-PCR:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Recombinant:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Expressing:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Electrophoresis:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Radio Immunoprecipitation:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Generated:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Immunodetection:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Protein Concentration:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    BIA-KA:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    SYBR Green Assay:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Centrifugation:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Stripping Membranes:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Real-time Polymerase Chain Reaction:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Western Blot:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Protein Purification:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Marker:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Acid Assay:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Binding Assay:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Bradford Protein Assay:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Transfection:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Polymerase Chain Reaction:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Recombinase Polymerase Amplification:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Blocking Assay:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Clarification Assay:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Transferring:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Saline:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Membrane:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    SDS Page:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Bicinchoninic Acid Protein Assay:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Chromatin Immunoprecipitation:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Ligand Binding Assay:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    RNA Sequencing Assay:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Lysis:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Imaging:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa

    Injection:

    Article Title: Membraneless protocell confined by a heat flow
    Article Snippet: schematic of the microfluidic geometry is shown in Supplementary Fig. . .. Sample loading with either full or amino-acid- and tRNA-deficient TX-TL reaction (PURExpress Delta aa, tRNA kit, E6840S, NEB) was conducted directly at the setup with low-pressure syringe pumps (neMESYS 290N, CETONI) mounted on a low-pressure 29:1 module (NEM-B101-03 A, CETONI).

    Article Title: The SNARE complex formed by RIC-4/SEC-22/SYX-2 promotes C. elegans epidermal wound healing.
    Article Snippet: Vazyme P525-AA I-5 23 High-Fidelity Master Mix MCLAB I5HM-200 ClonExpress II One Step Cloning Kit Vazyme C112-02 ClonExpress MultiS One Step Cloning Kit Vazyme Vazyme Gateway LR clonase II Invitrogen 56484 10X KLD Enzyme Mix NEB M0554S Polyacrylamide gel Kit (10%) epizyme PG112 SDS-PAGE Sample Loading Buffer (53) Biosharp BL502A Protein Ladder epizyme WJ103 Experimental Models: Organisms/Strains See Table S1 for a complete list of strains This pa



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    Image Search Results


    Journal: iScience

    Article Title: Fusobacterium species are distinctly associated with patients with Lynch syndrome colorectal cancer

    doi: 10.1016/j.isci.2024.110181

    Figure Lengend Snippet:

    Article Snippet: HiSeq Rapid Duo cBot v2 Sample Loading Kit , illumina , Cat# CT-403-2001.

    Techniques: Shotgun Sequencing, Recombinant, DNA Extraction, DNA Library Preparation, Electrophoresis, Software, Control, Sequencing